The International Microbiome Centre (IMC) Germ-Free Program is a core facility specializing in the development of germ free and gnotobiotic (known microbiome) animal models, with infrastructure and expertise for gnotobiotic research in healthy and diseased models.
Our vision is to harness the power of microbiome models for better living.
Our mission is to be leaders in microbiome germ free and gnotobiotic research through providing expertise, resources and service. IMC germ-free facility enables internal and external researchers, as well as industry partners to perform state-of-theart in vivo microbiome research using experimental conditions with precise microbial status. IMC expertise and resources in in vivo gnotobiotics allows for determination of mechanisms by which the microbiome causes chronic disease. The facility is setup with dedicated bereding and experimental rooms for performing germ-free, Level 1 and Level 2 gnotobiotic experiments. IMC is facilitating the development of a global network of expertise and service to enable collaborations and partnerships that will promote translation of microbiome research. IMC Germ-free facility will provide a basis for access to models, expertise and information for microbiome research.
Key Areas of Research
Microbiota in Chronic Diseases
Gnotobiotic experimental mice:
- Researchers and staff will receive training in gnotobiotic in vivo experimentation.
- A collection of inbred and outbred germ-free mice is available to researchers.
- Microbial colonizations with individual microbes, defined consortia, SPF, or human microbiotas are possible.
Axenic embryo transfer:
- We offer germ-free re-derivation of any mouse line through axenic two-cell stage embryo transfer.
- We offer contract research services for internal as well as external customers including industry partners.
- We offer consulting services for planning germ-free and gnotobiotic experiments as well as consulting for planning and designing a germ-free unit
Dr. Kathy McCoy, Scientific Director, International Microbiome Centre, Theme Lead, Germ Free Facility
Dr. Kathy McCoy is a Professor in the Cumming School of Medicine and Scientific Director of the International Microbiome Centre. She is also the Director of the germ free facility at the University of Calgary.Dr. McCoy is interested in the dynamic interplay between the gut microbiota and the innate and adaptive immune systems. Using germ-free and gnotobiotic mouse models her research group aims to understand how exposure to intestinal microbes early in life educates and regulates the developing immune system and how this impacts on susceptibility to immunemediated diseases such as allergy and autoimmunity.
Hebbandi et al. A Gut Microbial Mimic that Hijacks Diabetogenic Autoreactivity to Suppress Colitis. Cell. 2017 Oct 19;171(3):655-667
Mamantopoulos et al. Nlrp6- and ASCDependent Inflammasomes Do Not Shape the Commensal Gut Microbiota Composition. Immunity. 2017 Aug 15;47(2):339-348
Kwong et al. Detrimental effect of systemic antimicrobial CD4+ T-cell reactivity on gut epithelial integrity. Immunology. 2017 Feb;150(2):221-235
Zhang et al. Peripheral Lymphoid Volume Expansion and Maintenance Are Controlled by Gut Microbiota via RALDH+ Dendritic Cells. Immunity. 2016 Feb 16;44(2):330-42
Bomhof et al. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats. FASEB J. 2016 Aug;30(8):2720-32
McCoy et al. The Impact of Maternal Microbes and Microbial Colonization in Early Life on Hematopoiesis. J Immunol. 2018 Apr 15;200(8):2519-2526
McCoy et al. Host-microbiota interactions and adaptive immunity Immunol Rev. 2017 Sep;279(1):63-69
Lieu et al. iNKT Cells Orchestrate a Switch from Inflammation to Resolution of Sterile Liver Injury Immunity. 2017 Oct 17;47(4):752-765.e5.
Wang et al. Visualizing the function and fate of neutrophils in sterile injury and repair. Science. 2017 Oct 6;358(6359):111- 116.